Glycyrrhizic Acid And Its Derivatives As The Carriers For The Poorly Soluble Flavonoids

Abstract

Rutin is among the most explored and widely used flavonoids, but poor solubility limiting its usage triggers search for methods to improve its bioavailability. We offered to use glycyrrhizic acid and its derivatives as the carriers for rutin in supramolecular complexes.                Aim The work was initiated to comparatively study effects of two low dose rutin complexes, such as the one with glycyrrhizic acid (GA/rutin) and the one with monoammonium salt of glycyrrhisic acid (GAMS/rutin) in rats with the induced hyperlipidemia.

               Methods Rutin, as well as the GA/rutin and the GAMS/rutin complexes were administered to the animals per orally at the dose of 40 mg/kg for 10 days after induction of hyperlipidemia by Tween-80 (polysorbate 80).

               Results In the rats with the induced hyperlipidemia, the elevated total cholesterol (p<0.00001), triglycerides (TG) (p<0.00001), low density lipoproteins (LDL) (p<0.01), and malondialdehyde (MDA) (p<0.00001), as well as a reduction in blood catalase activity (p<0.05) and high density lipoproteins (HDL) was registered.  Rutin was found to reduce total cholesterol (p<0.01) and to increase catalase activity (p<0.05). The GAMS/rutin complex reduced total cholesterol (p<0.01), TG (p<0.001) and MDA (p<0.05), increasing HDL (p<0.05). The GA/rutin complex reduced total cholesterol (p<0.01), TG (p<0.0001), LDL (p<0.05) and MDA concentrations (p<0.01), increasing HDL (p<0.01) and catalase activity (p<0.05). The GA/rutin complex was found more efficient than rutin and the GAMS/rutin complex for some parameters under study.

               Conclusion as the result, the highly active supramolecular GA/rutin complex with GA and rutin ratio 4:1 called Biorutin has been chosen for further studies.