In vitro antibacterial, antioxidant and cytotoxicity response of CuO nanoparticles prepared by lemon juice and citric acid fueled solution combustion synthesis

Abstract

This research work presents the synthesis, characterization, evaluation of the antimicrobial and anticancer response of CuO nanoparticles prepared by solution combustion method using citric acid and lemon juice as fuels. The X-ray diffractograms of both the samples revealed the monoclinic structure of CuO with the standard JCPDS [45-0937]. Surface morphology of the samples was studied by scanning electron microscopy and transmission electron microscopy. Antimicrobial studies revealed the bactericidal and fungal activity of CuO nanoparticles. Furthermore, to better understand the parameters that affect the interactions between our CuO and mammalian cells, and thus their biocompatibility, we have examined the impact of cell culture conditions as well as of material properties on cytotoxicity by blood haemolysis, The results showed the biocompatibility of CuO NPs. 2, 2-diphenyl-1-picrylhydrazyl hydrate assay studies indicated the antioxidant activity of the samples at varied concentrations. Cytotoxicity studies revealed the anticancer activity of CuO NPs against PC-3, HCT116, A549 and MDA-MB-231 cancer cells.