The Role Of Intracellular Camp In The Production Of Glutathione From Rat Thymocyte Cells Under Hypoosmotic Stress

Abstract

Cyclic adenazine monophosphate acts as a secondary mediator involved in the intracellular signaling of certain hormones that cannot cross the cell membrane in the body. In our study, we studied the role of the adenylate cyclase signal cascade in glutathione output under hypoosmotic stress. 6.53 ± 0.15 μM of glutathione was isolated from 100 million / ml of cells for 20 min under control, and 4.45 ± 0.15 μM of glutathione was released when we observed the effect of 0.1 μM dibutyryl-cAMP (Membrane-permeable derivative of cAMP) solution on the same process (Figure 1A). In the next phase of our experiment, we increased the concentration of dibutyryl-cAMP to 1 μM, with 6,026 ± 0.1 μM of glutathione released under control and 5.65 ± 0.17 μM of glutathione under the influence of the substance. (Figure 1B). Hence, dibutyryl-cAMP significantly reduces glutathione release from thymocytes.  Activation of the adenylate cyclase system reduced glutathione release from thymocytes under hypoosmotic stress, and this result suggests that glutathione release under osmotic stress is organically linked to cAMP signaling pathways..