Measurement of Sensitivity and Oxygen of Blood by Amperometric Biosensor using GOX Enzyme for Detection of Cancer

  • Kalyan Babu, B. Keerthi Priya, B. Lakshmi Narayana Reddy, B. Ravi Chandra, A. Daisy Rani, D.V. Rama Koti Reddy


The detection of cancer is a tough task with limited components. The methods adopted in detection of cancer   are Biopsy, Biomarkers etc. Biopsy method involves invasive methods which takes longer time for detection of cancer. Biomarkers require   AFM, X rays, MRI scan for recognition of biomolecules which exhibit malignant behavior. Latest techniques like amperometric biosensor which is electro chemical in nature with Nano technology can detect cancer at an earlier stage within a stipulated amount of time. The output of amperometric biosensor is current which is the measure of oxygen gas which is very low in cancer living cell. Cancer cells are also referred to as Hypoxia cells where they inhale less amount of oxygen and release less amount of carbon dioxide in exhalation. In this paper detection of cancer is done through amperometric biosensor with one bulk solution and results are compared with another bulk solution. The best suited bulk solution with very high sensitivity to cancer cells is chosen as the final bulk solution for confirmation of cancer cell. An electro chemical method called Direct Current Cyclic voltammetry is used for detection of cancer. GOx enzyme   is used for diabetes detection, but in this paper, GOx is used for detection of cancer by calculating redox potentials. It is found that redox potential of cancer cell is very low in magnitude and output current falls due to low electron release in cancer cell.    GOx is mobilized and immobilized on the surface of the biosensor and the corresponding current is calculated. The bulk solution is the key solution of a biosensor for is effective working. The bulk solution is composed of potassium ferro cyanide, titanium dioxide, potassium nitrate and sulphuric acid.  The working of biosensor for detection of cancer is verified with different bulk solutions is verified and found that potassium ferro cyanide is best suited for detection of cancer with high sensitivity and oxygen level. This happened due to high electron binding affinity in cyanide compound, high molecular weight and volume..