Evaluation of Carica Papaya Linn. Fractions against DENV-2 Activity
Carica papaya Linn. (Caricaceae) has long been used as an alternative for folks medicine in the treatment of dengue fever in Malaysia. The aim of this study was to evaluate the in vitro activity from SPE methanolic-water fractions of Carica papaya leaves (CPL) against DENV-2 replication in C6/36 cells.
The CPL was fractionated by using SPE in methanol-water gradients. The antiviral activity of the fractions were assessed by employing qRT-PCR and the fractions were tested after the initiation of DENV-2 infection. The methanolic-water fractions inhibited up to 50% viral infectivity and the concentrations used were not cytotoxic to the cells. This study is the first evaluation of CPL fractions against DENV-2. The experimental data suggest that CPL fractions have drug ability as an anti-dengue agent.