Synthesis , Characterization & Biological Evaluation of Bio-fabricated Novel Copper Sulphide Nano particlesDerived From Copper (II) Complex

  • Sanchita Rai, Richa Kothari, Pradeep Mittal


Chalcogenide nanostructured  biologically active copper sulphide nanoparticles  (CuS) was prepared from 3-{(2E)-2-[1-(4-chlorophenylethylidene oxopropanamide thiosemicarbazone Copper (II) Chloride complex. This paper explains the biosynthesis of Copper sulphide nanostructures by using fresh leaves extract of Nyctanthes arbor tristis in stoichiometric ratio by a simple, fast, ecofriendly and inexpensive method. The crystal structure, physical properties and surface morphology of  copper sulphide nano particles  were characterized by UV-visible,FT-IR , H1 NMR,  powdered X-Ray diffraction (XRD), particle size distribution, Transmission Electron Microscopy and Scanning Electron Microscopy. The XRD analysis confirmed the synthesis of hexagonal crystal structure of CuS nanoparticles with the average particle size below 100 nm------ usingDebye-scherrer’s equation. The SEM analysis of   CuS nanoparticles have revealed that the agglomerated grains have a plate like structure composed of  copper sulphide nanoparticles. Formation of CuS nanoparticles were confirmed by UV-  visible spectroscopy. The FT-IR spectral study confirmed the presence of Cu-S stretching, N-H bending and C=N stretching vibrations. The thermal analysis of synthesized  Copper (II) complex  was perform to investigate the thermal stability of the copper complex . The thermal analysis was carried out from room temperature to 8000ֺC  in  an inert medium. The Cu complex and its  biosynthesized copper sulphide nanoparticles were  stable upto 160 0C.  The cytotoxicity test showed that the Cu complex and its biosynthesized nanoparticles were non toxic at low to moderate concentrations and only induced toxicity at higher concentrations. Cytotoxicity results revealed that the Copper complex  was less toxic compared to CuS nanoparticlkes as determined by their IC50 values .The antibacterial activity tests showed that the copper complex  exhibit more anti bacterial activity  as compared to its biofabricated copper sulphide nano particles as determined by  the serial dilution method.In vitro cytotoxicity studies and DNA fragementation studies showed that     showed that synthesized  copper complex was less toxic as compared to its biosynthesized copper sulphide nanoparticles against  A431 cancer cell  lines   . Anti bacterial activity of copper complex and its copper sulphide nanoparticles against  pathogens showed that copper complex has good activity as comparedto its bio fabricated copper sulphide nano particles.

How to Cite
Sanchita Rai, Richa Kothari, Pradeep Mittal. (2020). Synthesis , Characterization & Biological Evaluation of Bio-fabricated Novel Copper Sulphide Nano particlesDerived From Copper (II) Complex. International Journal of Advanced Science and Technology, 29(3), 11894 -. Retrieved from