Isolation, Characterization and Biofilm inhibition of Pyocyanin pigment extracted from Pseudomonas aeruginosa

  • Challaraj Emmanuel E.S., Madonna John, SiyamFathima

Abstract

Pseudomonas aeruginosa produce the pigment pyocyanin, which is a secondary metabolite and has antibiotic activity. Pyocyanin is a blue-green pigment that is produced by P. aeruginosa in a media that is devoid of iron content. Pyocyanin producing Pseudomonas aerginosa was isolated from soil sample.  Pyocyanin was extracted and purified using chloroform and then characterized using TLC, UV-Vis and FTIR scpectroscopy. The Rf value was found to be 0.79. The UV-Vis Spectrum showed a maximum peak at 277 nm. Pyocyanin has a high antimicrobial activity and can act as a broad-spectrum antibiotic. The inhibition concentrations were found to be as low as 5μg/ml.  The extracted pyocyanin showed antibacterial and anti-biofilm activities.

Published
2020-06-07
How to Cite
Challaraj Emmanuel E.S., Madonna John, SiyamFathima. (2020). Isolation, Characterization and Biofilm inhibition of Pyocyanin pigment extracted from Pseudomonas aeruginosa. International Journal of Advanced Science and Technology, 29(11s), 1772 - 1788. Retrieved from https://sersc.org/journals/index.php/IJAST/article/view/21700
Section
Articles