The Reduction Activity of Malondialdehide (Mda) Level in Combination of Ethanol Extracts of Moringa Leaf (Moringa Oleifera Lamk) and Clove Flower (Syzygium Aromaticum Merry & Perril)

Abstract

In pathological conditions such as diabetes mellitus, increased oxidative stress can cause an increase in malonaldehide (MDA) levels. Some sources of natural antioxidants are moringa leaf (Moringa oleifera Lamk) and clove flower (Syzygium aromaticum Merry & Perril). The purpose of this study was to determine the reduction activity of MDA levels in combination of ethanol extract of moringa leaf and ethanol extract of clove flowers. This study was an experimental laboratory study using a post test only group design. The subjects of this study were twentyfive male white rats of Wistar strain (Rattus norvegicus) which conditioned by diabetes mellitus with streptozotocin induction. Rats were randomly grouped into five groups and each group consisted of five rats. The dosage combination of ethanol extract of moringa leaf and clove flower used was 150:40 mg / kg BW of rats, 100: 80 mg / kg BW of rats and 50: 120 mg / kg BW of rats. The results showed that all combination of ethanol extract of moringa leaf (Moringa oleifera Lamk) and clove flower (Syzygium aromaticum Merry & Perril) had activities to reduce MDA levels. The average MDA level in the negative control was 9.10 ± 0.60 (nmol/g), while the best results was in the combination of ethanol extract of moringa leaves and clove flowers 150:40 mg/kg BW rats with an average MDA level of 1.57 ± 0.17 (nmol/g).