Krüpple-Like Factor 5 Has a Strong Correlation with Reversionless 3-Like in Cervical Cancer Cells
Cervical cancer is the fourth most frequent cancer in women caused by Human Papilloma Viruses (HPV) type 16 and 18 infections. A recent study suggested that HPV integration to the human genome can alter the expression of some genes, including KLF5. KLF5 plays a critical role in cell proliferation, an important process in cervical cancer pathogenesis. REV3L is believed to be involved in cell proliferation. This study aims to correlate KLF5 to REV3L in cervical cancer cells. This cross-sectional study was conducted in Trisakti Health Centre, Jakarta. Cervical tissues (6 normal and 4 cancerous tissues) were collected from 10 women. Normal tissues were obtained using the Pap smear technique, whereas cancer tissues were obtained using biopsy. All samples then transferred to a clinical laboratory for pathological testing and biomolecular laboratory for gene expression testing using qPCR. REV3L was upregulated in cervical cancer samples compared to normal (p<0.05) and it strongly correlated with KLF5 (R=0.787). There was no significant difference in the expression level of KLF5 between normal and cancer cells (p>0.05). There was a strong positive correlation between KLF5 and REV3L expression levels, suggesting that KLF5 and REV3L might affect each other in cell proliferation, which is a critical process in the development of cervical cancer. Taken together, KLF5 might be involved in regulating the expression of REV3L. These genes might have a critical role in the pathogenesis of cervical cancer cells.