“Identification of Fungus Species Isolated from Sandstone Monuments using RAPD-PCR”
Abstract
Aspergillus section Nigri include some of the most important species for biotechnology and its species are of widespread occurrence . Fungi play an major role in deterioration of sandstone. The capability of Fungus in production of pigments and organic acids have pivotal role in discoloration and degradation of different types of sandstone in cultural heritage. Secondary, sandstone objects may support novel biocommunities that affected area active in biodeterioration process. Furthermore, mycelia of fungus can impale into the internal structure of sandstones and change the interior chemical composition of stones. This study was focused on the identification of fungi that were colonized on the sandstone monument. The samples were collected from different monument places built during different time periods and analyzed using cellophane tape methods and swabbing & serial dilution methods. Sampling of stone was carried out to identify inhabiting microorganism. biochemical and microscopic methods were used for isolated strains of samples. In addition, the Polymerase Chain Reaction (PCR) and sequencing of the PCR products were done. Finally, phylogenic tree was constructed base on the sequences of ITs region We identify high microbial diversity fungal groups. The fungal commodity structure differed among the samples. The inhabiting fungi which isolated from sandstone monuments belong to high microbial diversity fungal groups. The results revealed that the strain exhibited a high level of 18S rRNA similarity with Aspergillus costaricaensis , Aspergillus luchuensis Aspergillus aflatoxiformans isolate DTO 228-G2, Rhizopus sexualis var.americanus, Rhizopus oryzae CBS 111.07 was identified as a close relative to Aspergillus . The RAPD PCR techniques seems to be practical and well organized for routine use in high resolution microbial analysies by giving assemblage comparisons through sequence information proving or fingerprinting.
