Possibilities of using Long Bone Marrow in Autogenous Therapy

Abstract

New high-tech medical advances, especially cellular technology, offer prospects for treatments with a more pronounced therapeutic effect. Previously, all studies were conducted on flat (red) bone marrow – sternal puncture or the iliac crest. Studies of long bone marrow were not performed due to the impossibility of obtaining it in vivo. The present study aims at quantitative analysis of multipotent mesenchymal stromal cells (MMSC) and hematopoietic stem cells (HSC) in bone marrow samples of amputated limbs in patients of different age groups.
Materials and methods: long bone marrow samples were obtained from 35 patients who underwent lower limb amputation due to gangrene as a result of ischemia, traumatic injuries, road accidents, hypothermia; the bone marrow was removed from the femur immediately after the surgery. The number of HSC and MMSC was determined on a flow cytometer using a set of antibodies to surface antigens of human cells. Immunohistochemical characterization of bone marrow cells was made for all the samples.
Results:the number of MMSC in bone marrow samples was 0.50-0.90% (average value 0.20 ± 0.04%), and the number of HSC was 0.01-0.90% (average value 0.27 ± 0.05%). Due to the high content of cells in question (in other words, biological value) and relative simplicity of the surgical procedure of obtaining long bone marrow, MMSC and HSC can be used in autogenous therapy for various diseases.
Key words: limb amputation, bone marrow, autogenous cell therapy, stem cells, cell transplantation, therapeutic strategy