In-Vitro Cytotoxic and Anti - Angiogenesis Studies of Polyvinyl Alcohol Mediated 5fluorouracil Conjugated Iron Oxide Nanoparticles

Abstract

The objective of the researchis toevaluate the in-vitro cytotoxic and anti angiogenesisactivity of 5-fluorouracil conjugatedironoxidenanoparticlesmediatedwithPolyvinylalcohol. Ironoxide Nano particleswerechemicallysynthesised and conjugatedwith 5 –Fluorouracildrug. This mixture wasthenadded to Polyvinylalcohol. This PVA isact as a drug carrier vehicle. Characterization of this compound werecharacterizedusing UV, FTIR, Thermo-Gravimetric/Differential Thermal Analysis (TG/DTA), Raman Spectroscopy, Field Emission Scanning Electron Microscope (FESEM) and X-ray powder diffraction (XRD). 5-Fluorouracil drug is Nano conjugated with these synthesized PVA coated iron oxide nanoparticles.When a drug is bound to iron –oxide nanoparticles, their tolerance can be overcome due to a reduction of the efflux pumps that transport the drug to the outside of the cell and promotes an increase in drug concentration in cancerous tissues.The result shows the effectiveness of the sample in increasing the angiogenesisactivity (i.e) formation of new bloodvesselscompared to the standard pyruvicacid. The resultsclearly show the effectiveness of the drug in treatingcancerouscells by deprivingcells of nutrients and oxygenbecause cancer cellsrequireaccess to bloodvessels for growth and metastasis.This non-toxicity effect of this drug also makes it more effective for the treatment of various types of cancer and reduces the mortality and morbidity from carcinoma. The increased anticancer activity was found even at lower concentration of the drug. Thus, synthesized nanoparticles were more efficient in the biomedical applications in cancer treatment for their high anticancer activity.

 Keywords : Anti-cancer, 5 fluorouracil, ironoxide, nano particle, chitosan, CAM assay