In-vivo Anti-arthritic Evaluation of Simhanada guggulu in FCA Induced Arthritis in Rats
Abstract
AIM- The main aim of the present investigation is to study In-vivo Anti-arthritic Evaluation of Simhanada guggulu in FCA Induced Arthritis in Rats. MATERIAL & METHODS- Adult female and male Wistar rats of weight 150- 200 g, which were bred and reared in the department animal house, were used for this study. The acute oral toxicity studies were carried out according to the guidelines set by the Organization for Economic Co-operation and Development (OECD), revised draft guideline 423. The Wistar albino rats were divided into 10 groups of six animals in each. For the induction of chronic inflammatory response, FCA (0.1 ml) was injected through intra-articular injection in left ankle joint of rats on 0 day. Body weight was measured of all groups at zero days before immunization and at 21st day after treatments over by using a single pan weighing balance. At the end of the experimental period, rats were fasted overnight and the blood sample were collected by retro orbital blood sampling techniques from anaesthetized rats. On the last day of study, samples of plasma were sending to the Pathology Lab, for estimation of TNF-alpha levels. The Estimation was done with the help of Elisa Reader in Pathology Lab. RESULTS- No toxic effects were observed at a higher dose of 2000 mg/kg body weight of Wistar rats. Hence, 1/ 10th dose was selected as effective dose or therapeutic dose. The assessment made on the 21st day showed that the Simhanada guggulu treatments at both doses (low and high) had moderately significant and highly significant effect and reduced (p< 0.01& p<0.001) the adjuvant-induced lesions in the respective treatment groups as compared with the arthritis control group. MDA levels were observed to increase in Group II when compared with Group I. However, GSH levels and SOD activities were observed to decrease in Group II when compared with Group I. Administration of Simhanada guggulu caused highly significant decrease (p<0.001) in MDA levels and increase in GSH and SOD activities however. Treatment with Simhanada guggulu showed a highly significant decrease at both the doses (p<0.001) as compared to the arthritic rats. Prednisolone (10 mg/kg) treated rats also showed significant decrease in TNF-α levels. CONCLUSION- In conclusion, it can be stated that the Simhanada guggulu have beneficial effects in long lasting reduction in rat paw edema, arthritic index and various lysosomal enzymes.
