Development & Validation Of Rp-Hplc Method For Estimation Of Glabridin In A Novel Formulation

Abstract

Glabridin is a bioactive phytoconstituent present in licorice (Glycyrrhiza glabra Linn). The glabridin-loaded transfersomal gel was developed to treat a variety of hyperpigmentation disorders. Following successful preparation, The HPLC process has been developed and validated to study glabridin in transfersomal formulation. The HPLC separation was carried out on a Kromasil C8 column (250 mm X 4.6 mm, 5m) with acetonitrile & acetate buffer (70:30 v/v) as a mobile phase and a flow rate of 1 ml/min. Quantification was performed at a detection wavelength of 228 nm. In compliance with the International Conference on Harmonization standards, the procedure is validated for accuracy, precision, reproducibility, robustness, and identification, as well as quantification limits. According to statistical analysis, the approach is precise, reproducible, selective, and effective for the study of glabridin. The proposed developed and validated high-performance liquid chromatography system for quantifying glabridin can control and standardize licorice and various herbal formulas containing licorice.