WSSV TRANSMISSION EXPERIMENTS ON POLYCHAETE PERENERIS CULTIFERA TO FENNEROPENAEUS INDICUS IN CAPTIVITY
Abstract
The present study was White spot syndrome virus (WSSV) collected from Polychaete -Perinereis cultrifera which was orally transmitted with white spot syndrome virus (WSSV) and conformed with PCR identification is transferred to Fenneropenaeus indicus by intramuscularly injection and through oral rout.100% mortality of Fenneropenaeus indicus was resulted on 13th day in oral rooted and on 15th day for intramuscularly injected shrimp. Mortality rate of 90.0 and 93.30 % were observed during 12 and 14 days of post infection of WSSV in Fenneropenaeus indicus treated by oral route and intramuscularly. Whilst the appearance of a prominent band of PCR amplified product of WSSV-DNA at 650 bp and 296 bp continued from day 3 to 9. On days 8, 9, 10 and 14 the product band observed as 650 bp. The product band of 296 bp, 650 bp and 910 bp were noticed during 11, 12, 13 and 15 days of post-inoculum respectively. No evidence of DNA band observed in the control group. Hence it is conclude that the immunity of F.indicus is more against pathogens while comparing with other Phenaied shrimps. This result that F.indicus can survive more days than other shrimps. The histopathological analysis in hematopoietic tissue on the fifth day of post inoculated shrimp shows ranges on low level infection. The results from the study strongly suggest developing specific pathogen resistant brooders would immensely useful in rearing of shrimp for commercial purposes. Even though, shrimp hatcheries in India and other Asian countries depend almost entirely all natural polychaete stocks, continuation of while polychaete population with lethal pathogen such as WSSV demonstrate the need to produces pathogen free polychaete worm especially P.cultrifera through aquaculture. However, by implementing quarantine prior bring P.cultrifera commercial shrimp breeding purposes will immensely boot the industry.
