Effect of Lupeol in Diabetic Nephropathy and Its Anti-oxidant Mechanism
Abstract
AIM- Effect of Lupeol in Diabetic Nephropathy and Its Anti-oxidant Mechanism. MATERIAL & METHODS- Lupeol was purchased from Himedia Pvt Ltd., Mumbai. Animals were fasted for overnight then a single intra peritoneal injection of freshly prepared Streptozotocin (50 mg/kg dissolved in citrate buffer pH 4.5) was injected and Nephropathy was induced by Ligation of Left Renal Artery. Blood was collected on 0, 14 and 28 day by retro-orbital on 28 days from eye for the estimation of biochemical parameters. The withdrawn blood was centrifuged at 5000 rpm for 10 min by using centrifuge and plasma was collected and different biochemical parameters were determined by autoanalyzer. Some antioxidant parameters i.e. MDA, GSH, Catalase and SOD were also determined. RESULTS- Here body weight of rat is decreased in Diabetic Nephropathy group in comparison to Normal Control group while increased significantly in Lupeol group at 28th day in comparison to Diabetic Nephropathy group. Here Blood glucose level of Diabetic Nephropathy group was increased in comparison to Normal Control group while decreased significantly in lupeol group at 14th, 21st & 28th day in comparison to Diabetic Nephropathy group. CONCLUSION- The overall results of this study have clearly indicated that Lupeol a dose of 35 mg/kg ameliorate the renal ischemia reperfusion induced nephropathy in diabetic rats. The action of Lupeol contributed by renoprotective, anti-diabetic, antioxidant properties of Alstonia scholaris. Further studies are needed to stabiles possible mechanism of action for protective effect of Lupeol in Alstonia scholaris.
