Dual Property of Tamarind Seed Polysaccharide Aid Wound Healing

Abstract

Amongst several wound natural wound healers, Xyloglucan (XG) are being extensively studied for their healing property. XG, proved to enhance cell viability, proliferation and migration in human skin cells, cure wounds individually or in combination. Together with its property of hydrogel production and film formation, it aids slow drug delivery, acting as a wound enhancer, meanwhile. Tamarind seed polysaccharide (TSP) was extracted from Tamarind seed kernel powder, using a protocol involving consecutive, boiling, centrifuging and precipitation steps. Identification of XG from Tamarind seed polysaccharide (TSP) was performed using Liquid chromatography-Mass spectrometer (LC-MS/MS), Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (NMR) studies. Scratch assay conducted on TSP treated human fibroblast cells at 0 hr., 5 hr. and 24 hr. time period, reveal significant differences in scratch area due to migration of skin fibroblast cells, in comparison with non-treated and fetal bovine serum (positive control), confirming its potency in In-vitro wound healing.