LACCASE GENE FAMILY PROFILING IN DIFFERENT MUSHROOM VARIETIES
Abstract
In the present study gene expression of laccase enzyme in fungal species, Pleurotus ostreatus, Ganoderma lucidum and Morchella esculenta was determined.SDS analysis of crude extract of all three species showed a good protein expression near 48KDa MW of ladder specifically in Pleurotus ostreatus.Qualitative analysis of DNA which included DNA isolation, PCR amplification, gel electrophoresis and subsequent staining predicted highly purified DNA in Morchella esculenta followed by Pleurotus ostreatus and Ganoderma lucidum. PCR amplification of Laccase gene showed good gene expression.