Electromagnetic Field Effects on antibacterial activity of Jasminum grandiflorum L.

Abstract

Amongst different environmental factors, electromagnetic radiation of different wavelengths Viz. PAR (Photosynthetically active radiation) have profound effects on synthesis of bioactive compounds and overall herbal yield of medicinal plants. Other radiations like UV-B (Ultraviolet-B) radiation was also investigated extensively for its capability to modulate synthesis of various secondary metabolites (like phenolic and terpenes) responsible for medicinal properties of plants. But there is no such investigation was commenced which can explain the effect of high frequency waves on physiological and biochemical properties of such medicinal plant. Thus, the presented research was held in the thirst of investigating modulation in antimicrobial potential of Jasminum grandiflorum L. In which the experimentation was governed by analysis of antimicrobial activity of selected plant extract against six pathogenic bacterial strains such as Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhimurium, E.coli, Serratia marcescens and Bacillus subtilis. The experiment was governed by standard Broth dilution method and Agar diffusion method whose results indicated significant variation in Minimum Inhibitory Concentration (MIC) and Inhibition zone diameter (IZD) of electromagnetically unexposed and exposed plant extracts. The maximum bactericidal effect of this selected plant extract was reported in case of Bacillus subtilis Strain which was 17±0.41 mm of IZD and 0.60±0.019 µg/ml MIC and least potency was reported against Pseudomonas aeruginosa strain which was 14±0.45 mm IZD and 0.87±0.039 µg/ml MIC. The significant (p˂0.05) variation in MIC and IZD was reported upon exposure of radiation for 36 hours which was 0.78±0.013 mm and 13±0.23 µg/ml respectively, in case of strain Bacillus subtilis (against which highest bactericidal effect was obtained) which can be considered as detrimental effect when compared with the electromagnetically unexposed control plants. It revealed 30% of decrease in antibacterial activity upon 36 hours of radiation of 1800 MHz frequency. Bactericidal effects against other bacterial strains also shown prominent deteriorative effect upon and after 36 hours.