AN INITIATION TOWARDS IDENTIFYING A NEW CHEMICAL ENTITY TO TREAT CANCER BY HOMOLOGY MODELLING AND DOCKING OF COLCA2

Abstract

Colorectal cancer is a cancer of colon or rectum, it’s a deadly disease and occurs majorly due to proliferation.GWAS is an observational study of genetic variants for different individuals to identify variants in a trait, the associations between the single-nucleotide polymorphisms (SNPs) and also major human diseases. COLCA2-colorectal cancer associated gene 2 is a protein coding gene that is involved in various molecular functions, biological processes. Homology modeling is the prediction of resolution model of the "target" protein from the sequence of amino acid based on the experimental three-dimensional structure of a related homologous protein using Galaxyseok lab server which is based on template based modeling(TMB). The protein model obtained from the above server was then checked for quality using the Rampage server. This server gives a plot called the Ramachandran plot which shows the quality of the models. Peptide-protein interactions are very prevalent for most of the biological processes such as signal transduction, protein trafficking and etc. Peptide protein docking was done from cabs dock. Interaction of theGGST and GGTS peptide were observed.. Interaction of the GGST peptide where seen those residue((Receptor residue,Peptide residue)(TYR A 153,THR B 4)(ASP A 150,SER B 3)(TYR A 143,GLY B 1)(GLU A 62,SER B 3)(PRO A 61,GLY B 2)(PRO A 14,THR B 4)(ILE A 151,THR B 4)(TYR A 143,GLY B 2)(ASN A 63,GLY B 1)(GLU A 62,GLY B 1)(PRO A 57,THR B 4)(TYR A 153,SER B 3)(ASP A 150,GLY B 2)(TYR A 64,SER B 3)(GLU A 62,GLY B 2)(SER A 58,THR B 4)).Interaction of GGTS peptide where seen those residue((Receptor residue,Peptide residue)(TYR A 153,THR B 3)(ASP A 150,GLY B 2)(THR A 139,GLY B 1)(ASN A 63,GLY B 2)(GLU A 62,GLY B 2)(PRO A 14,SER B 4)(ALA A 13,THR B 3)(ASP A 150,THR B 3)(TYR A 143,GLY B 1)(TYR A 64,GLY B 1)(GLU A 62,THR B 3)(HIS A 15,SER B 4)(ALA A 13,SER B 4)(ILE A 151,THR B 3)(ASP A 150,GLY B 1)(TYR A 64,GLY B 2)(ASN A 63,GLY B 1)(PRO A 57,SER B 4)(PRO A 14,THR B 3)).