Fractionationof α/β-Carotene and β-Cryptoxanthin in Red Fruit Oil (Pandanus conoideus Lamk) Using Flash Column Chromatography Methods

  • Hendra Wijaya, Lukman Junaidi, Rudi Heryanto, Ning Ima Arie Wardayanie, Nami Lestari, Fitri Hasanah, Santi Ariningsih, Rhoito Frista Silitonga, Dedy Darmawan Samid, Samuel Pieter Baliony Sangkoyo

Abstract

Carotenoids have a function as a good antioxidant that is often used as a supplement. Red fruit oil is a potential sourceof carotenoid. This study aims to optimize the carotenoid fractionation process from red fruit oil (Pandanus conoideusLamk) using the flash column chromatography (FCC) method. Fractionation of carotenoids in oil samples includes oil characterization, carotenoid extraction from samples assisted by saponification reaction, carotenoid fractionation with FCC, characterization of carotenoids by thin layer chromatography (TLC), UV-Vis spectrophotometer, and highperformance liquid chromatography (HPLC). Product moisture content is 0.34% and extract yield is 5.80% with total carotenoids 1110 μg/g. TLC test result using extract from hexane: ethyl acetate solvent (9:1)with eluent hexane: acetone (6: 4)(7: 3) (8: 2) and ( 9: 1) produce 4, 5, 4, and 3 traces respectively. Then the extract from hexane: ethyl acetate solvent (9:1)was fractionated with FCC using Gradient 1 and Gradient 2. Identification with UV-Vis and HPLC spectrophotometers showed that Gradient 1 produced a dominant fraction containing α/β-carotene and theβ-cryptoxanthin fraction can be produced from gradient 2.

Keywords: red fruit, fractionation, carotenoids, flash column chromatography.

 

Published
2020-03-30
How to Cite
Hendra Wijaya, Lukman Junaidi, Rudi Heryanto, Ning Ima Arie Wardayanie, Nami Lestari, Fitri Hasanah, Santi Ariningsih, Rhoito Frista Silitonga, Dedy Darmawan Samid, Samuel Pieter Baliony Sangkoyo. (2020). Fractionationof α/β-Carotene and β-Cryptoxanthin in Red Fruit Oil (Pandanus conoideus Lamk) Using Flash Column Chromatography Methods. International Journal of Advanced Science and Technology, 29(3), 9313 - 9327. Retrieved from http://sersc.org/journals/index.php/IJAST/article/view/26536
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Articles