Evaluation of isolated gut probiotic Bacillus subtilis on Haematological parameters of Indian major carp Labeo rohita (Hamilton, 1822)

Abstract

The research study followed out to determine the influence of isolated single gut probiotic bacteria Bacillus subtilis (SUB 3845847 SeqJP2 MH128358) on the haematological parameters of freshwater carp Labeo rohita. After acclimatization, fish (5.00 cm ± 4.5 gm) were allocated into the tubs such as a control tub (feed without B. subtilis) and an experimental tub (feed fed with B. subtilis) was continued, 30 fingerlings fish brought together keen on the separate tub. The formulated diet such as ricebran 40%, groundnut oil cake 20%, dry fishmeal 15%, soyameal 15%, maize 9%, vitamins and minerals mix 1% were analyzed for the proximate composition. The formulated diet was given each day morning at 6 am and evening at 6 pm usually at the ratio of three percent of body weight. The water was to exchange each day with a pump and every treatment had triplicates. The blood samples were collected on fifteen days intervals of the experimental period in 60 days. The results exposed that the haematological parameters were insignificantly (P<0.05) increased in the experimental group than the control group. The isolated gut probiotic B. subtilis with formulated diet increased the haematological values of the haemopoietic stimulation in river carp Labeo rohita.