Effect of Murraya Koenigii & Datura Stramonium L. on Splenocyte Proliferation
Abstract
The aim of this study is to evaluate and validate the Immunomodulating potential of medicinal plants Murraya koenigii and Datura stramonium L. which are known in Ayurvedic literature for the remedy of various alignments. Chemical constituents present in the leaves of Murraya koenigii and Datura stramonium, accounted for its anti-cancer, anti-nociceptive, anti-bacterial, anti-diabetic, anti-diarrheal, cytotoxic, anti-ageing, anti-oxidant, nephroprotective and lipid lowering activities. Immunomodulating activity was analyzed by splenocyte proliferation assay. The plant extracts were prepared through hot aqueous extraction method using soxhlet apparatus. Spleen cells were isolated from albino Wistar rats. The effect of these plant extracts on cell mediated immune responses was evaluated by splenocyte proliferation assay. Spleen cells were cultured in the presence of different concentration of both the plant extracts i.e.25, 50, 100, 250, 500µg/ml in the presence of 10µg/ml ConA mitogen. Extract of Murraya koenigii stimulated the proliferation of splenocyte and a maximum proliferation of 42.37% was obtained with 100µg/ml of Murraya koenigii while 50µg/ml of Datura stramonium extract showed maximum proliferation of 23.76% of spleen cells. At higher concentration Datura stramonium extract showed cytotoxic effect.
