Anti-Fungal Bioactivity of Gaddi Sheep’s Milk Oligosaccharide

Abstract

Carbohydrate is a natal component of all living beings like: bacteria, fungi, plants and placental mammals’ milk, to which oligosaccharide are the vital integredient. The milk is a fluent source of oligosaccharides with various novel oligosaccharides depends on the nature of the origin of mammals the milk belongs. The basic role for milk is to support the immune system of a newborn, and provide bioactive molecules that allow the gastrointestinal tract of the new born to mature. A normal part of this purulence is the development of a healthy balance of microflora. Goat milk oligosaccharides have anti-inflammatory effects. It is useful for intestinal protection and repair after the damage caused by DSS (Dextron sodium sulphate)-induced colitis and their implication in human intestinal inflammation. Buffalo milk oligosaccharides have resistivity contrary to parasitic infections. Donkey milk oligosaccharides have potentiality to stimulate non-specific and specific immunological resistance. The cows’ milk oligosaccharides diminish the adhesion of enterotoxic Escherichia coli strains in calf. Recalled all biological activities of different milk in mind, Gaddi sheep's milk was collected and processed by Modified method of Kobata and Ginsburg and then it was purified by Sephadex G-25 Gel column. The homogeneity was confirmed by reverse phase high performance chromatography. Acetylation of mixture of oligosaccharides processed from the silica gel chromatography direct to segregation of novel oligosaccharides. The fungal activity of Gaddi sheep’s milk oligosaccharides was investigated against different fungus namely Aspergillus brasiliensis ATCC 16404, Aspergillus fumigatus ATCC 9197, Trichophyton ajelloi ATCC 28454 and Fusarium solani ATCC 36031, on the basis of their relevance as human pathogens. All samples of milk oligosaccharide exhibited fungal activity against these fungal species.