Evaluation of Some Biochemical Parameters in Relation to Obesity in Male Rats

Abstract

Objective: This study aimed to investigate the link between metabolic changes, antioxidant enzymes, and oxidative stress induced by obesity. Materials and Methods: The study was carried out on 20 male Sprague Dawley. Rats were administered orally with MSG at a dosage level (15 mg/kg B.W for three months). Bodyweight, triacylglycerol, total cholesterol, fasting blood glucose, and HbA1c – insulin, calculated HOMA-IR, liver enzymes, creatinine, urea, oxidative stress marker, as well as histopathological examination of hepatic and renal tissue were determined. Results: MSG obese rats showed a highly significant increase in average body weight, triacylglycerol, total cholesterol, fasting blood glucose, HbA1c, insulin, HOMA-IR, liver enzyme, and kidney function, and malondialdehyde (MDA) compared to the control. A highly significant reduction in glutathione (GSH), superoxide dismutase (SOD), and catalase in hepatic and renal tissues compared to the control. Histopathological alterations were determined in the kidney and liver tissues of obese rats. Conclusion: The findings of this research reveal that MSG in this dose led to obesity that results in insulin resistance besides oxidative stress, and histopathological alterations determined in renal and hepatic tissues.