Microbial Profile of Bacteriuria in Pregnant Women and their Antibiogram
Abstract
Bacteriuria refers to the presence of bacterial contaminants in urine when bacterial load is above 105 CFU/ml. In the present study routine microbial investigation was carried out on 450 urine samples of pregnant women collected from Patna Medical College, Bihar, India. 350 cases of asymptomatic and 100 of symptomatic bacteriuria infections were identified though routine microbial investigation. The bacteriuria was found to be also influenced by the socio-economic status of the population with prevalence among population living under compromised sanitation.
Isolated bacterial cultures were identified using different biochemical test in accordance to the Bergey’s manual for microbial identification. E. coli (56.6%), Klebsiella pneumoniae (15.09%), Staphylococcus (Coagulase ‘-’) (9.43%), Proteus mirabilis (7.54%), Staphylococcus aureus (5.66%), Pseudomonas aeruginosa (3.77%) and others were isolated. Antibiotic sensitivity tests were carried out for all bacterial isolates using standard disc diffusion (Kibry Bauer) method. The sensitivity of different antibiotics varied against the test microorganisms with 81.35% of amikacin (10 mcg) against E. coli; 12% nitrofurantoin (10 mcg) against Klebsiella pneumoniae. Meropenem (10 mcg) displayed effective antibacterial activity against Staphylococcus (coagulase ‘-’), S. aureus, Pseudomonas spp. and Proteus spp. with sensitivity of 72.72%, 57.14%, 66.66% and 25% respectively. In contact to the individual antibiotic activity, synergistic effect of antibiotics especially cefoperazone and sulbactum (10 mcg each) was found to be highest. Study indicates that antibiotics displaying synergistic action must be employed for the early treatment and recovery from UTI infection minimizing the chances of abortions.
