Investigating the effects of medicinal smoke on CA125 and HE4 biomarkers on ovarian cancer cell line

Abstract

Background: Ovarian cancer is the most lethal gynecologic cancer in women. This cancer is caused by various factors such as mutagenic substances and carcinogenic chemicals in the environment. Overexpression of some genes including CA125 and HE4 is involved in ovarian cancer. CA125 tumor marker is considered a tumor index to confirm disease diagnosis, determine the extent of the disease and evaluate the response to treatment and application in non-mucinous epithelial ovarian tumors. Human epididymis protein 4 (HE4) has been approved for the evaluation of disease or progression of ovarian epithelial cancer. The medical smoke from natural resources is used in many countries around the world. One of these medicinal smokes is the smoke from burning the female donkey dung called Anbar Nesara. Anbar Nesara can be used as a useful drug for treating diseases such as sinusitis, cold and cervical and vaginal diseases.

Material and method:Anbar Nesara contains large amounts of organic materials and nitrogen and also plant materials consumed by the animal. MTT test was performed by the triplicate method. Then, the effect of CA125 and HE4 biomarkers on cells was evaluated.

Results:The results of MTT‏ assay demonstrated that the cells of both lines show a significant dose-dependent decrease in CA125 and HE4 gene expression in treatment withAnbar Nesara, which may be a reason for the tumor suppressive effect through apoptosis.

Conclusion:These suppressive effects of Anas on apoptotic responses of CA125 and HE4 on A2780 cell line can be due to the presence of a set of herbal compounds used. Identifying the precise mechanism of these effects and determining the apoptotic role of these compounds require further studies.